Primary Cell Culture: The Insider's Guide to Media Selection and Common Traps
Primary cells are isolated directly from living tissues (human, mouse, monkey, etc.) via enzymatic digestion or mechanical dissociation, then cultured in vitro for the first time. As a critical ...
Huh‑7: The Workhorse Cell Line for Liver Cancer and Hepatitis Research
Among liver research models, few have achieved the status of Huh‑7. Derived from a well‑differentiated hepatocellular carcinoma (HCC), this cell line has become indispensable for studying viral ...
Human Primary Cells — What You Should Know?
Primary cells specifically refer to cells that are isolated or harvested directly from living tissue or organs. With the exception of stem cell and progenitor populations, the majority of primary ...
Overview of Cell Separation
Cell separation is the process of removing one cell population from another within a biological sample, such as blood or tissue. It was first achieved in 1964 by S. H. Seal, who separated large tumor ...
Overview of T Cell Activation and Expansion
In recent years, the boom in immune cell therapy has led to an increased interest in the industrial production of T cells. To accomplish successful cell therapy, a large number of CAR-T or other ...
Peripheral Blood Mononuclear Cells — What You Should Know?
Peripheral Blood Mononuclear Cells (PBMCs) are any blood cell with a single nucleus. These blood cells are a critical component in the human immune system to fight pathogens. PBMCs consist of ...
iPSC: Characteristics, Culture, Differentiation
Induced pluripotent stem cell (iPSC) is a pluripotent stem cell that is introduced to a series of inducing factors into mature somatic cells and reprogrammed into a type of pluripotent stem cell with ...
Preparation and Applications of Buffy Coat
In a sample of peripheral whole blood, less than 1% of the cells are white blood cells (WBCs) and platelets. When researchers put the sample through a centrifuge, a machine that spins the blood, ...
Neural Stem Cells: Source, Culture, Identification, Differentiation
Neural stem cells (NSCs) are primitive cells with self-renewal and multi-differentiation potential that are preserved during the development of the nervous system. With the deepening of the ...
Cell Counting Techniques
Cell culture is a critical step in cell biology research. To ensure healthy cell growth and facilitate downstream experiments, researchers must determine cell growth curves and understand the growth ...