Cell Line Development
With current advances in technology and biopharmaceutical research, cell line development (CLD) has become a prominent process in many application areas. One of these areas would be the development ...
iPSC: Characteristics, Culture, Differentiation
Induced pluripotent stem cell (iPSC) is a pluripotent stem cell that is introduced to a series of inducing factors into mature somatic cells and reprogrammed into a type of pluripotent stem cell with ...
Magnetic Cell Separation
Cell isolation techniques are methods to separate and transfer certain cells from a complex mixture of cells to obtain single cells or to sort the cells according to a property of choice and thus ...
Neural Stem Cells: Source, Culture, Identification, Differentiation
Neural stem cells (NSCs) are primitive cells with self-renewal and multi-differentiation potential that are preserved during the development of the nervous system. With the deepening of the ...
Hematopoietic Stem and Progenitor Cells — What You Should Know?
Hematopoietic stem/progenitor cells (HSPCs) isolated from bone marrow have been successfully employed for 50 years in hematological transplantations. Currently, these cells are more frequently ...
Overview of Organoid Research
Organoids are a complex collection of cells grown in a three-dimensional (3D) medium, which are derived from embryonic stem cells (ESC), induced pluripotent stem cells (iPSC), or adult stem cells ...
Preparation and Applications of Buffy Coat
In a sample of peripheral whole blood, less than 1% of the cells are white blood cells (WBCs) and platelets. When researchers put the sample through a centrifuge, a machine that spins the blood, ...
Overview of Cell Separation
Cell separation is the process of removing one cell population from another within a biological sample, such as blood or tissue. It was first achieved in 1964 by S. H. Seal, who separated large tumor ...
Cell Counting Techniques
Cell culture is a critical step in cell biology research. To ensure healthy cell growth and facilitate downstream experiments, researchers must determine cell growth curves and understand the growth ...
Peripheral Blood Mononuclear Cells — What You Should Know?
Peripheral Blood Mononuclear Cells (PBMCs) are any blood cell with a single nucleus. These blood cells are a critical component in the human immune system to fight pathogens. PBMCs consist of ...