HOW WE ACHIEVE CELL IMMORTALIZATION

Research is, and will always be, our primary source of discovery. The use of cells in research projects to determine the various effects of a multitude of stimuli is crucial in the scientific process of learning and evaluation. Cell immortalization will play an increasingly vital role in research today and in coming decades. And providing this very specific and technical service requires years of experience in working with the various processes.

The successful immortalization of various cells; human, bovine, dog, and mice, is implemented using different genes and agents. Cell immortalization can be done in many ways; and Creative Bioarray’s custom method is able to extend the replicative capacity of your targeted cells significantly past their natural replicating ability; saving our clients, innumerable amounts of time and finances.

It is widely known that primary cells have a finite number of replications. Cells cease to divide after undergoing a predetermined number of life spans. This natural process is called senescence. Researchers can choose to attempt the recreation of fresh cultures from explanted tissue; a time consuming and tedious process which may or may not be successful. Utilizing a cell immortalization service helps researchers avoid this issue and offers them the possibility of an almost limitless supply of cells that can be used throughout the duration of an entire research project. Consistent cells will often provide more accurate discoveries.

There are many methods of mammalian cell immortalization within culture environments. We will briefly define a few of these methods below:

Virus Induction: EBV, HPV E6/7 and SV40 T Antigen

This process of cell immortalization utilizes viral genes; the Simian Virus 40 T antigens, EBV, and the HPV-16 E6/7 gene. The Simian Virus 40 T antigens have been proven to be one of the simplest ways to immortalize cells and one of the most reliable agents for transforming different types of cell culture.

hTERT Expression

A newly discovered approach to cell immortalization is through the expression of Telomerase Reverse Transcriptase Protein, otherwise known as TERT. This recently discovered process is most successful for cells affected by telomere length, which are normally human cells. The process enables cells to maintain sufficient telomere lengths to avoid senescence.

Inactivation of Tumor Suppression Genes

Recent discoveries have found that co-expression of hTERT catalytic subunit combined with p53 or RB siRNA, can successfully immortalize human ovarian epithelial cells. These results allow a more authentic cell model with a more defined genetic background.

Creative Bioarray provides researchers with primary cells, along with a guarantee that cells are successfully immortalized. We confidently work with various types of cells, including; liver, lung, ovarian, brain, pancreas, prostate, skin, thoracic aorta, and umbilical cord.  Our cell immortalization projects are overseen and conducted by experienced scientists with short turn-around times; ensuring our clients will receive a quality service that will aid significantly in the success of their current research projects.